If you mean if the selector is in 3rd then yes, it should. The only thing that I know of that is disabled (different from when it is in "D" but in 3rd gear) is AFM, that will not work if the selector is not in "D"
Also it is possible that you have a different tranny than mine. I think that the trans makes less heat when the TC is locked so maybe if it is a 4l60e then they do it to cut down on heat?? Mine is a 4l70e. Just a guess on that one.